Generator

Part:BBa_K2207011:Design

Designed by: Yuxing Chen   Group: iGEM17_ZJU-China   (2017-10-29)


phlO-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1384
    Illegal AgeI site found at 1496
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The site of the phlo region relative to the ADH1 promoter, Kozak sequence


Source

Mailed plasmid and biobrick part

References